Assessment of Gene Expression during Osteoblast Induction of Human Mesenchymal Stem Cells
نویسندگان
چکیده
منابع مشابه
[Assessment of gene expression during osteoblast induction of human mesenchymal stem cells].
The aim of this study was to define transcriptional changes that occur during dexamethasone-induced in vitro osteoblastic differentiation using human mesenchymal stem cells (hMSCs). Bone marrow derived hMSCs from three individual donors were grown in DMEM containing 10% fetal bovine serum and antibiotics (basal media, BM). At confluence (Day 0), cells were grown in BM or BM plus ascorbic acid, ...
متن کاملGene Expression Profile of Adherent Cells Derived From Human Peripheral Blood: Evidence of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) provide a novel option in cellular therapy and tissue engineering. Recent studies indicated that it is possible to obtain MSCs from peripheral blood by attachment ability to plastic surface. To evaluate adherent cells derived from peripheral blood, their expression profile and surface markers were investigated. The results of RT-PCR indicated that these cells expre...
متن کاملThe Effects of Iron Oxide Nanoparticle on Differentiation of Human Mesenchymal Stem Cells to Osteoblast
Introduction: IIron oxide nanoparticles (IO NP) have an increasing number of biomedical applications. To date, the potential cytotoxicity of these particles remains an issue of debate. Little is known about the cellular interaction or toxic effects of IO NP on differentiation of stem cells. The aim of the present study was to investigate the possible toxic role of different doses of IO NP in di...
متن کاملIn Vitro Assessment of the Gene Expression of EZH-2 and P300 During Motor Neuron Differentiation of Human Umbilical Cord Blood Mesenchymal Stem Cells
Introduction: Maintenance of neurogenesis depends on the function of some histone-modifying enzymes; including Enhancer of zeste homolog 2 (EZH2) and histone acetyltransferases (P300). The mechanism of epigenetic regulation and gene expression underlying the transition of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) into MNs has not been fully clarified. Methods: Two morphoge...
متن کاملMesenchymal Stem/Stromal-Like Cells from Diploid and Triploid Human Embryonic Stem Cells Display Different Gene Expression Profiles
Background: Human ESCs-MSCs open a new insight into future cell therapy applications, due to their unique characteristics, including immunomodulatory features, proliferation, and differentiation. Methods: Herein, hESCs-MSCs were characterized by IF technique with CD105 and FIBRONECTIN as markers and FIBRONECTIN, VIMENTIN, CD10, CD105, and CD14 genes using RT-PCR technique. FACS was performed fo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN
سال: 2004
ISSN: 1884-5185,0300-9149
DOI: 10.5357/koubyou.71.11